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I had 13 samples taken and only one hurt....but it hurt big time. Blood in semen for 6 weeks. There was virtually no pain having the prostate removed...but I'll never forget that pain from the biopsy. I thought I read somewhere that biopsies weren't used much anymore as some sort of imaging was more accurate with less side effects and less risk. Perhaps I'm mistaken.
For some odd reason the effects of a biopsy are almost always downplayed. I guess it is the same reasoning that they use to not inform you of the loss of length, girth, and firmness of your dysfunctional penis after a prostatectomy. Poking a dozen core holes through your rectal tissue and into a small organ like the prostate is a major trauma for both rectal and prostate tissue. The damage is obvious as witnessed by the immediate short term blood loss and also the length of time there is blood in the semen. Pain runs the gambit from none to plenty.